Title
Effect of indole-3-acetic acid, kinetin, and ethylenediaminetetraacetic acid on plant growth and uptake and translocation of lead, micronutrients, and macronutrients in alfalfa plants
Publication Date
2-1-2009
Publication Name
International Journal of Phytoremediation
Document Type
Article
Abstract
Alfalfa plants germinated and grown for 15 d in soil containing 80 mg Pb kg-1 were treated with ethylenediaminetetraacetic acid (EDTA) at 0.8 mM and indole-3-acetic acid-kinetin (IAA-KN) at 100 μM. Fifteen days after the treatment application, the concentration of lead (Pb), macronutrients, and micronutrients was determined using inductively coupled plasma/optical emission spectroscopy. The chlorophyll content and plant growth were also measured. Roots of plants exposed to Pb alone, Pb-EDTA, and Pb-EDTA-IAA-KN had 160, 140, and 150 mg Pb kg-1 DW, respectively. Pb was not detected in the stems of plants exposed to Pb alone; however, stems of plants treated with EDTA and EDTA-IAA-KN had 78 and 142 mg Pb kg-1 DW, respectively. While the Pb concentration in leaves of plants treated with EDTA and EDTA-IAA-KN was 92 and 127 mg kg-1 DW, respectively. In addition, EDTA and EDTA-IAA-KN significantly increased the translocation of zinc and manganese to leaves. The x-ray absorption spectroscopic studies demonstrated that Pb(II) was transported from roots to leaves without a change in the oxidation state. Copyright © Taylor & Francis Group, LLC.
Volume
11
Issue
2
First Page
131
Last Page
149
DOI
10.1080/15226510802378434