Date of Award
2016-01-01
Degree Name
Master of Science
Department
Physics
Advisor(s)
Chunqiang Li
Abstract
Two-photon microscopy is a fluorescence imaging technique which provides distinct advantages in three-dimensional cellular and molecular imaging. The benefits of this technology may extend beyond imaging capabilities through exploitation of the quantum processes responsible for fluorescent events. This study utilized a two-photon microscope to investigate a synthetic photoreactive collagen peptidomimetic, which may serve as a potential material for tissue engineering using the techniques of two-photon photolysis and two-photon polymerization. The combination of these techniques could potentially be used to produce a scaffold for the vascularization of engineered three-dimensional tissues in vitro to address the current limitations of tissue engineering. Additionally, two-photon microscopy was used to observe the effects of the application of the neurotransmitter dopamine to the mushroom body neural structures of Drosophila melanogaster to investigate dopamine's connection to cognitive degeneration.
Language
en
Provenance
Received from ProQuest
Copyright Date
2016
File Size
54 pages
File Format
application/pdf
Rights Holder
Kevin Andrew Hatch
Recommended Citation
Hatch, Kevin Andrew, "Two-Photon Excitation Based Photochemistry and Neural Imaging" (2016). Open Access Theses & Dissertations. 859.
https://scholarworks.utep.edu/open_etd/859
Included in
Biophysics Commons, Optics Commons, Physical Chemistry Commons