Date of Award

2017-01-01

Degree Name

Master of Science

Department

Metallurgical and Materials Engineering

Advisor(s)

Namsoo Peter Kim

Abstract

Tissue in particular extracellular matrix(ECM) plays a vital role in the growth and the function of each cell types and promote tissue regeneration and repair an impair organs. Many techniques have been developed so far to decellularized porcine tissue for the purpose to use as regenerative medicine and in tissue engineering. We aimed to optimized a method to decellularized porcine tissue while effectively eliminating xenogeneic epitopes, lipids and to retain necessary component of it intact without disrupting ECM. We have developed 2-week protocol to optimize well preserved ECM component that may keep xenogeneic biomaterial for tissue engineering and regenerative medicine. Porcine adipose tissue was first thaw, minced and then homogenized in ultrapure water followed by chemical, physical and enzymatic treatments. After the decellularization protocol, material was freeze-dried for further analysis. With a significant reduction, trifle amount of inherent immunogenic components such as cells and nucleic acid were recorded using Histology (H&E staining) and DNA quantification. However, significant ECM component such as collagen, glycosaminoglycan and elastin were determined and found well preserved. The entire analysis and research on porcine adipose tissue using 14-day protocol have suggested that the method to decellularized ECM derived from porcine adipose tissue could be useful one with little modification in the protocol to optimize the method. we assure that the method to obtain extracellular matrix is efficient for cell removal and preserved the collagen which may possessed the most favorable biocompatibility and most befitting material for tissue engineering and regenerative medicine.

Language

en

Provenance

Received from ProQuest

File Size

49 pages

File Format

application/pdf

Rights Holder

Rahulkumar Kantibhia Bhoi

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