Date of Award

2024-05-01

Degree Name

Master of Science

Department

Materials Science And Engineering

Advisor(s)

Wilson Poon

Abstract

Nanoparticles are currently known to be a promising material class for bio-applications in drug delivery and vaccine development. Using gold nanoparticles of varied sizes, in this case 45 and 100 nanometers as a model nanomaterial system, we investigated how patients' blood physiology and chemistry (such as solute, protein, lipid levels) affect the biological response to bionanomaterials. When nanoparticles are injected into the body, biomolecules in the blood adsorb to the nanoparticle's surface to form a biomolecular corona that is specific to the patient's unique blood composition. This biomolecular corona is important because it affects the in vivo fate and biodistribution of the administered nanoparticles Here we show that the abundance of certain proteins and lipids in patient blood changes the biomolecular corona of nanoparticles of the varied sizes. Furthermore, the presence of these proteins and lipids affected in vitro cell uptake of nanoparticles. Our preliminary results suggest that certain blood composition biomarkers can predict patient-specific biomaterial interaction. Future experiments will follow up on these candidate protein and lipid biomarkers and investigate if they are applicable in predicting nanoparticle interactions of different physicochemical properties, such as lipid-based nanoparticles. Studying how patient-specific blood biomarkers interact with biomaterials can help us rationally design personalized nanomaterials for therapeutic applications to improve their health.

Language

en

Provenance

Received from ProQuest

File Size

54 p.

File Format

application/pdf

Rights Holder

Veronica Gabriela Contreras

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Biomedical Commons

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