Date of Award
2017-01-01
Degree Name
Master of Science
Department
Physics
Advisor(s)
Chunqiang Li
Abstract
Two-photon fluorescence microscopy is an imaging technique which delivers distinct benefits for in vivo cellular and molecular imaging. Cyclic adenosine monophosphate (cAMP), a second messenger molecule, is responsible for triggering many physiological changes in neural system. However, the mechanism by which this molecule regulates responses in neuron cells is not yet clearly understood. When cAMP binds to a target protein, it changes the structure of that protein. Therefore, studying this molecular structure change with fluorescence resonance energy transfer (FRET) imaging can shed light on the cAMP functioning mechanism. FRET is a non-radiative dipole-dipole coupling which is sensitive to small distance change in nanometer scale. In this study we have investigated the effect of dopamine in cAMP dynamics in vivo. In our study two-photon fluorescence microscope was used for imaging mushroom bodies inside live Drosophila melanogaster brain and we developed a method for studying the change in cyclic AMP level.
Language
en
Provenance
Received from ProQuest
Copyright Date
2017-08
File Size
67 pages
File Format
application/pdf
Rights Holder
Syeed Ehsan Ahmed
Recommended Citation
Ahmed, Syeed Ehsan, "Imaging Live Drosophila Brain With Two-Photon Fluorescence Microscopy" (2017). Open Access Theses & Dissertations. 397.
https://scholarworks.utep.edu/open_etd/397