Date of Award

2013-01-01

Degree Name

Master of Science

Department

Chemistry

Advisor(s)

Katja Michael

Abstract

Amino acid and peptide oxo-esters have played an important role in peptide chemistry for many years. Various amino acid esters are commonly used as protecting groups for peptide synthesis in solution. Moreover, activated phenyl ester derivatives have been used for chemical ligation of peptides. However, the synthesis of amino acid and peptide oxo-esters is commonly performed under acidic or basic conditions. Under acidic conditions, acid-sensitive protecting groups may be cleaved, and under basic conditions racemization of amino acid esters is a common problem. Here we present the photochemical synthesis of amino acid and peptide oxo-esters. Based on the photochemical properties of N-acyl-7-nitroindolines, photoacylation is performed to produce amino acid and peptide esters under neutral conditions, thus minimizing racemization or epimerization. We demonstrated the usefulness of this method by installing ester protecting groups, by synthesizing fairly activated phenyl esters, fluorescent amino acid esters, and a fluorescent TAT peptide ester.

Language

en

Provenance

Received from ProQuest

File Size

142 pages

File Format

application/pdf

Rights Holder

Alfredo Ornelas

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