Title
Sculpting MHC class II-restricted self and non-self peptidome by the class I antigen-processing machinery and its consequences on TH cell responses
Publication Date
4-25-2013
Document Type
Article
Abstract
It is generally assumed that the MHC class I antigen (Ag)-processing (CAP) machinery —which supplies peptides for presentation by class I molecules— plays no role in class II-restricted presentation of cytoplasmic Ags. In striking contrast to this assumption, we previously reported that proteasome inhibition or TAP- or ERAAP-deficiency led to dramatically altered T helper (TH) cell responses to allograft (HY) and microbial (Listeria monocytogenes) Ags. Herein, we tested whether altered Ag processing and presentation, altered CD4+ T cell repertoire, or both underlay the above finding. We found that TAP- and ERAAP-deficiency dramatically altered the quality of class II-associated self peptides suggesting that the CAP machinery impacts class II-restricted Ag processing and presentation. Consistent with altered self peptidomes, the CD4+ T cell receptor repertoire of mice deficient in the CAP machinery substantially differed from that of wildtype animals resulting in altered CD4+ T cell Ag recognition patterns. These data suggest that TAP and ERAAP sculpt the class II-restricted peptidome, impacting the CD4+ T cell repertoire, and ultimately altering TH cell responses. Together with our previous findings, these data suggest multiple CAP machinery components sequester or degrade MHC class II-restricted epitopes that would otherwise be capable of eliciting functional TH cell responses.
Comments
Spencer, C. T., Dragovic, S. M., Conant, S. B., Gray, J. J., Zheng, M. , Samir, P. , Niu, X. , Moutaftsi, M. , Van Kaer, L. , Sette, A. , Link, A. J. and Joyce, S. (2013), Sculpting MHC class II–restricted self and non‐self peptidome by the class I Ag‐processing machinery and its impact on Th‐cell responses. Eur. J. Immunol., 43: 1162-1172. doi:10.1002/eji.201243087