Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy

Publication Date


Document Type

Conference Proceeding


Bin Cao, Sayan Chakraborty, Wenqing Sun, Seyedmohammadali Aghvami, Fischer MG, Wei Qian, Chuan Xiao, Chunqiang Li. Imaging marine virus CroV and its host cafeteria roenbergensis with two-photon microscopy. 2014;8944:89440E,8944-6.


We use two-photon microscopy to monitor the infection process of marine zooplankton, Cafeteria roenbergensis (C.roenbergensis), by Cafeteria roenbergensis virus (CroV), a giant DNA virus named after its host. Here, we image C.roenbergensis in culture by two-photon excited NADH autofluorescence at video-rate (30 frame/s), and the movement of C.roenbergensis is recorded in live videos. Moreover, CroV is stained with DNA dye SYBR gold and recorded simultaneously with this two-photon microscope. We observed the initial infection moment with this method. The result demonstrates the potential use of two-photon microscopy to investigate the fast dynamic interaction between C.roenbergensis with virus CroV. After catching this initial moment, we will freeze the sample in liquid nitrogen for cryo-electron microscopy (EM) study to resolve the virus-host interaction at molecular level. The long-term goal is to study similar fast moving pathogen-host interaction process which could lead to important medical applications.