Date of Award
Doctor of Philosophy
Charles T. Spencer
This dissertation investigates the impact of Francisella tularensis infection on the brain, focusing on its anatomical localization, effects on synaptic health, and behavioral and cognitive consequences in mice. Employing a combination of fluorescent tagging, confocal and light sheet microscopy, the study first maps bacterial foci within the brain, anticipating a concentration in the limbic system, particularly the hippocampus. Subsequently, it examines synaptic function post-infection using calcium imaging in various neuronal cultures, expecting a decrease in calcium signaling indicative of synaptic impairment. Finally, behavioral assays like the forced swim test, object recognition test, and Morris Water Maze assess the cognitive and behavioral effects of the infection, hypothesizing a disruption in hippocampal function leading to impaired memory and learning. This comprehensive study aims to elucidate the mechanisms of neural disruption caused by Francisella tularensis, contributing to a deeper understanding of its neurological impacts and potential therapeutic avenues.
Recieved from ProQuest
Tena, Anahis, "Neural Damage Following Francisella Tularensis Infection" (2023). Open Access Theses & Dissertations. 4025.
Available for download on Thursday, January 16, 2025