Date of Award
Doctor of Philosophy
Arshad M. Khan
Rodent models are invaluable for understanding the foundations of motivatedbehaviors. One major obstacle faced by these efforts is the lack of a 'wiring diagram' or a 'parts list' of structures that support motivated behaviors. Here, I present work that advances our knowledge of the structural organization of connections and chemoarchitecture of the diencephalon. Chapter 1 aims to clarify a wiring diagram of the medial prefrontal cortex (mPFC) following a recent demonstration of cortically-evoked feeding through this region. Chapter 2 clarifies the distributions of a subpopulation of tyrosine hydroxylase (TH) neurons in the hypothalamus. These disparate datasets were analyzed and interpreted using a Nissl-based mapping approach and nomenclature system that facilitates construction of a spatial model for motivated behaviors. Using this methodology, I describe widespread and topographically organized reciprocal connectivity between mPFC and thalamus as well as subregion-specific connectivity with the hypothalamus. I also demonstrate heterogeneity among hypothalamic TH neurons on the basis of their co-expression of the vesicular GABA transporter. Taken together, this work describes a general principle of mPFC connections with thalamus that has been overlooked, and it contributes important insights toward a comprehensive wiring diagram and parts list for feeding and other motivated behaviors.
Recieved from ProQuest
Negishi, Kenichiro, "Connectional analysis of brain regions associated with feeding" (2023). Open Access Theses & Dissertations. 3835.