Supercritical Processed Decellularized Extracellualr Matrix as Regeneration Therapeutics Applying 3D Printing
Extracellular matrix has been broadly applied and show great promise in medical applications and tissue engineering. Product of extracellular matrix (ECM) should be treated with decellularization and purification process. Supercritical carbon dioxide treatment is of particular interest for purifying ECM because of its medically available and rapid speed of process. However, it is not fully researched in treatment of biomaterials for tissue engineering. Therefore, we investigated optimal conditions of supercritical carbon dioxide processing in different extracting parameters from porcine adipose tissue. The 3-day, dual treatment including enzymatic decellularization and supercritical fluid extraction of pork adipose tissue were performed. Two types of protocols using different extracting parameters were applied in order to evaluate the influence of extracting pressure, and temperature on the extraction yield, DNA concentration and remaining collagen amount of product. Yield rate was increased when higher temperature or pressure were applied, and pre-enzyme treatment had higher percent of yield rate than the pre-supercritical processing. DNA removed nearly 90% when extracted at highest pressure (3.04×107Pa) and low temperature (30±5℃) in the pre-enzyme sample. As for collagen concentration, pre-enzyme process showed efficient extracting ability at each temperature and pressure. Remaining collagen steadily decreased with increasing of extracting pressure and temperature. At the lowest temperature (20±5℃) and lowest pressure (1.01×107Pa), remaining collagen was 75.74±1.83%. Supercritical extraction technology can produce DE-ECM eliminating DNA content efficiently and remaining proper collagen amount successfully. This study evaluated the feasibility of utilizing supercritical extraction technology with bio-materials and it is proven that it is successful. Through this technology of controlling extracting pressure and temperature, it has a potential for the DE-ECM mass production can be useful as tissue regeneration therapeutics as well new drug delivery paradigm. To improve bio-compatibility, 3D printing technologies (piston type of extrusion method) are applied to fit for bio-environment and this thesis purpose, and material processing for producing printable bio-ink was conducted. PTE method was successfully applied to print 2D pattern for medical patch application and 3D structure for implantable application.
Materials science|Biomedical engineering
Chung, Seungwon, "Supercritical Processed Decellularized Extracellualr Matrix as Regeneration Therapeutics Applying 3D Printing" (2020). ETD Collection for University of Texas, El Paso. AAI27829036.