Cu(II) Lipoplexes Of 1,4,7-Triazacyclononane And Their Biological Properties

Juan C. Noveron, University of Texas at El Paso


In order to aid in the design of future oligonucleotide transport vehicles we synthesized an array of surfactants and test their differences in transfection efficiency. A novel class of surfactant-copper (II) complexes, Cu(TACN-C8)2, Cu(TACN-C10)2, Cu(TACN-C12)2, Cu(TACN-C14)2, Cu(TACN-C16)2, and (TACN-C18)2, were synthesized and fully characterized to generate, through self-assembly, cationic nanoparticles. Supramolecular characterization of nanoparticles and nanopartilce/DNA condensates was conducted through CMC, DLS and microscopy. An evaluation of the transfection efficiency of each surfactant was assessed by transformation of a Hela cell line, for which the highest observed efficiency was 38% corresponding to the surfactant with the dodecyl alkyl chain.