Date of Award

2016-01-01

Degree Name

Master of Science

Department

Physics

Advisor(s)

Chunqiang Li

Abstract

Major innovations in recent years have largely revolutionized the fluorescence imaging. Two-photon fluorescence microscopy is one of them. Two photon fluorescence microscopy has evolved as an alternative to conventional single-photon confocal microscopy and has been shown to provide several advantages. These include three-dimensionally resolved fluorescence imaging of living cells deep within thick, strongly scattering samples, and reduced phototoxicity, enabling long term imaging of photosensitive biological specimens. The inherent three-dimensional resolution of TPE microscopy has been exploited in a number of studies wherein spatial discrimination of fluorescence signals at the micrometer and submicrometer scale within thick biological specimens proved critical. In this study, we used our two-photon microscope to observe grazing behavior of fast moving marine organism Cafeteria roenbergensis with the interaction of bacteria.

Language

en

Provenance

Received from ProQuest

File Size

65 pages

File Format

application/pdf

Rights Holder

Faisal Abedin Abedin

Included in

Physics Commons

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