A PDMS/paper hybrid microfluidic device integrated with graphene oxide-based nano-biosensors for multiplexed pathogen detection
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
We have developed a polydimethylsiloxane (PDMS)/paper hybrid microfluidic system integrated with aptamerfunctionalized graphene oxide (GO) nano-biosensors for one-step multiplexed pathogen detection. The paper substrate used in this hybrid microfluidic system facilitated the integration of nano-biosensors on the chip, and avoided complicated surface treatment and aptamer probe immobilization in a PDMS or glass-only microfluidic system. Lactobacillus acidophilus was first used as a bacterium model to develop the microfluidic platform, with a limit of detection of 11.0 cfu/mL obtained. We have further successfully extended this method to multiplexed detection of two infectious pathogens-Staphylococcus aureus and Salmonella enterica.