Possible interactions between phospholipase A(2)S and COX-2 in colon carcinoma cell line, HT-29
Hyper-arachidonic acid metabolism in colonic epithelial carcinoma cells has been linked to the increased production of inflammatory agents that promote tumorigenesis, angiogenesis, and other hallmarks of cancer cells. Although both secretory phospholipase-A2 (sPLA2) and cytoplasmic phospholipase-A2 (cPLA2) were shown to be involved in releasing arachidonic acid (AA) from arachidonoyl-phospholipads (AA-PL) during malignancy, nothing is known regarding their interplay at the cellular level. Through microarray and quantitative RT-PCR analyses, it has been shown that short-term (4 hours) administration of AA (25 μM/106 cells) in HT-29 cells results in upregulation of a multitude of genes including Ca2+ dependent PLA2 (cPLA2), secretory PLA2 (sPLA2), several oncogenes and protein kinases. The inhibition of sPLA2 activity by aristolochic acid (ArA, 160 μM/10 6 cells) reduces the expression of cPLA2, Rab 7 and Jun messages but increases COX-2 transcription. (Abstract shortened by UMI.)
Macias, Sandra G, "Possible interactions between phospholipase A(2)S and COX-2 in colon carcinoma cell line, HT-29" (2005). ETD Collection for University of Texas, El Paso. AAI1430241.